Supplementary MaterialsFigure S1: Differentially transcribed genes (DTGs) in the colon adenocarcinoma cell line, Caco-2, upon interaction with GS isolate for 1. the combined band of selected genes for differentiated Caco-2 cells. Picture_2.TIF (949K) GUID:?278ACAB3-D6F8-4198-A44F-B10B83047AE2 Body S3: Immunofluorescence pictures teaching the nuclear recruitment of nuclear aspect kappa-B (NFB) in the differentiated colon adenocarcinoma cell line, Caco2, upon co-incubation with GS isolate trophozoites. The green fluorescent dots in the nucleus represent NFB. Picture_3.TIF (2.7M) GUID:?662D9B7E-31DF-4E33-9112-E5026FE4C096 Body S4: The translocation of nuclear aspect kappa-B (NFB) in to the nucleus of proliferating digestive tract carcinoma cell series, Caco-2, upon co-incubation with GS isolate. Defense staining displays green fluorescent dots in the nucleus representing NFB. Proliferating Caco-2 cells incubated with 100 ng of tumor necrosis aspect alpha per ml of lifestyle moderate represent the positive control within this test. Negative control is certainly proliferating Caco-2 cells incubated by itself in culture moderate. Picture_4.TIF (3.2M) GUID:?4F9AD848-0929-4730-8024-A93844C95ED4 Body S5: High temperature map teaching temporal adjustments in the RNA degrees of genes connected with response to oxidative tension and cell routine regulation in the differentiated digestive tract carcinoma cell series, Caco-2, co-incubated with GS isolate. The fold transformation in RNA amounts is provided for three co-incubation period factors (1.5, 3, and 4.5 h). Picture_5.TIF (282K) GUID:?9C6D8114-669A-4B47-BDCC-A93C92960E56 Desk S1: Differentially transcribed genes (DTGs) in the differentiated digestive tract adenocarcinoma cell series, Caco-2, in response to interaction with isolate, GS, for 1.5 h. Remember that considerably up-regulated genes are in crimson font whereas considerably down-regulated genes are in blue font (altered 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Desk S2: CB-7598 cost Differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, in response to interaction with isolate, GS, for 3 h. Remember that considerably up-regulated GENES are in crimson font whereas considerably down-regulated genes are DSTN in blue font (altered 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Desk S3: Differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, in response to interaction with isolate, GS, for 4.5 h. Remember that considerably up-regulated genes are in crimson font whereas considerably down-regulated genes are in blue font (altered 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Desk S4: Differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, in response to interaction with isolate, GS. DTGs had been identified upon an evaluation of Caco-2 cells transcriptomes at 3h versus 1.5h. Remember that considerably up-regulated genes are in CB-7598 cost crimson font whereas considerably down-regulated genes are in blue font (altered 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Table S5: Differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, in response to interaction with isolate, GS. DTGs had been identified upon an evaluation of Caco-2 cells transcriptomes at 4.5 vs. 3 h. Remember that considerably up-regulated genes are in crimson font whereas considerably down-regulated genes are in blue font (altered 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Desk S6: Differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, incubated with GS isolate for 1.5, 3, and 4.5 h. The desk displays overlapping genes in the three period factors (orange-colored cell) and DTGs particular to each relationship time CB-7598 cost factors (1.5 h green, 3 blue and 4.5 h yellow). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Table S7: Gene ontology (GO) analysis for the differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, upon interaction with GS isolate for 1.5 h. The desk displays an enrichment of Move functions connected with natural procedure (BP), molecular procedure (MP) and mobile component (CC) ( 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Table S8: Gene ontology (GO) analysis for the differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, upon interaction with GS isolate for 3 h. The desk displays an enrichment of Move functions connected with natural procedure (BP), molecular procedure (MP) and mobile component (CC) ( 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Table S9: Gene ontology (GO) analysis for the differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, upon interaction with GS isolate for 4.5 h. The desk displays an enrichment of Move functions connected with natural procedure (BP), molecular procedure (MP) and mobile component (CC) ( 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Table S10: Gene ontology (GO) analysis for the differentially transcribed genes (DTGs) in the differentiated colon adenocarcinoma cell series, Caco-2, upon interaction with GS isolate. The Move conditions are enriched upon evaluating Caco2 cell transcriptome between 3 and 1.5 h. The desk displays an enrichment of Move functions connected with natural procedure (BP), molecular procedure (MP) and mobile component (CC) ( 0.05). Desk_1.XLSX (16M) GUID:?82B8AF3E-2201-497C-B997-6C17F2BE1CC8 Abstract is.