Supplementary MaterialsAdditional file 1 The Six MIAME guidelines that were adopted to conduct the study. CCs. 1471-2164-12-57-S9.XLS (1.6M) GUID:?C5587489-F401-49ED-A482-FE68BD716899 Additional file 10 The top significantly changed GO (molecular functions) with the proportion of transcripts that are over expressed in GV oocytes and CCs (P 0.001). 1471-2164-12-57-S10.TIFF (171K) GUID:?11BC3EBC-8BA8-4EBC-A889-7B015DD103EF Additional file 11 The top significantly changed GO (molecular functions) with the proportion of transcripts that are over expressed in MII oocytes and CCs (P 0.001). 1471-2164-12-57-S11.TIFF (140K) Belinostat irreversible inhibition GUID:?7051C98E-3FE8-42BC-B102-15087939C93C Additional file 12 List of transcripts that are differentially expressed between oocytes cultured with or without their companion CCs. 1471-2164-12-57-S12.XLS (69K) GUID:?17DDB623-CFB3-4376-A9BA-60935FDE1729 Additional file 13 List of transcripts that are differentially expressed between CCs cultured with or without their oocyte. 1471-2164-12-57-S13.XLS (121K) GUID:?2915768A-2D41-48BE-A56F-0C19151647D9 Additional file 14 The seven most prominent canonical pathways involving genes that are over expressed in OO + CCs relative to OO – CCs with P-values, 0.05. The bars represent the P-value for each pathway. The orange irregular line is usually a graph of the ratio (genes from the data set/total quantity of genes involved in the pathway) for the different pathways. 1471-2164-12-57-S14.TIFF (93K) GUID:?44C7D188-5BCE-4FB6-A4D7-20CCC66BB4BE Additional file 15 The five most prominent canonical pathways involving genes that are over expressed in CCs + OO relative to CCs – OO with P-values, 0.05. The bars represent the P-value for each pathway. The orange irregular line is usually a graph of the ratio (genes from the info set/total variety of genes mixed up in pathway) for the various pathways. 1471-2164-12-57-S15.TIFF (62K) GUID:?DB633D3A-B278-4B60-B9B8-CA756D088136 Additional file 16 Set of transcripts that are expressed in GV CCs in comparison to MII Belinostat irreversible inhibition CCs exclusively. 1471-2164-12-57-S16.XLS (563K) GUID:?AE6F4D64-4B5D-413C-8F27-B05467400AE6 Additional document 17 Set of transcripts that are expressed in MII CCs weighed against GV CCs exclusively. 1471-2164-12-57-S17.XLS (116K) GUID:?66F6F3FE-30A5-442A-A65A-6CE5F394991C Extra file 18 Set of transcripts that are portrayed between CCs at GV and MII stages commonly. 1471-2164-12-57-S18.XLS (885K) GUID:?AED914A6-88D4-4E01-B49C-2D89E3B31B24 Additional document 19 Set of transcripts that are expressed between GV and MII CCs differentially. 1471-2164-12-57-S19.XLS (870K) GUID:?B3A394E8-4244-4127-A903-1B0B4E682819 Extra file 20 qRT-PCR validation from the microarray data showing the change in the expression degrees of transcripts differentially portrayed between GV oocytes and CCs. Belinostat irreversible inhibition Transcripts proclaimed with the minus indication indicate those over portrayed in GV CCs. 1471-2164-12-57-S20.DOC (34K) GUID:?1C529862-7FF2-4356-BB88-C9867C2A6ECC Extra file 21 qRT-PCR validation from the microarray data showing the change in the expression degrees of transcripts differentially portrayed between MII oocytes and CCs. Transcripts proclaimed with the minus indication indicate those over portrayed in MII CCs. 1471-2164-12-57-S21.DOC (31K) GUID:?BB89EBD1-628B-41C1-AE75-925360A54579 Additional file 22 qRT-PCR validation from the microarray data showing the transformation in the expression degrees of transcripts differentially portrayed between oocytes cultured using their companion CCs in accordance with those cultured alone. Transcripts proclaimed with the minus sign indicate those over expressed in oocytes cultured without their companion CCs. 1471-2164-12-57-S22.DOC (33K) GUID:?3F4FD846-12CD-4786-B6F4-B87C9034279C Additional file 23 qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between CCs cultured with their enclosed oocytes relative to those cultured alone. Transcripts marked by the minus sign indicate those over expressed in CCs cultured without their enclosed oocytes. 1471-2164-12-57-S23.DOC (34K) GUID:?916D3A81-3210-4B4D-835B-A29508D45A62 Additional file Belinostat irreversible inhibition 24 qRT-PCR validation of the microarray data showing the switch in the expression levels of transcripts differentially expressed between CCs at germinal vesicle (GV) and metaphase II (MII) stages. Transcripts marked by the minus sign indicate those over expressed in MII stage relative to GV stage. 1471-2164-12-57-S24.DOC (31K) GUID:?0664103B-414D-437F-8275-324A07E43FEF Abstract Background The bi-directional communication between the oocyte and its companion cumulus cells (CCs) is crucial for development and functions of both cell types. Transcripts that are exclusively expressed either in oocytes or CCs and molecular mechanisms affected due to removal of the communication axis between the two cell types is not investigated at a larger scale. Rabbit polyclonal to PCSK5 The main objectives Belinostat irreversible inhibition of this study were: 1. To identify transcripts expressed possibly in oocyte or CCs and 2 solely. To identify those that are expressed when the oocyte is cultured differentially.