Supplementary MaterialsS1 Fig: Composition and the morphology of the cellularity in BALF. which received 50 uL of PBS intranasally; C) 0.5 mg/g HBG extract treated mice; D) 0.1 mg/g HBG extract treated mice with undamaged alveolar space and parenchymal architecture, E) mice treated with 0.1 mg/g of 6-bromoisatin, and; F) mice treated with 0.05 mg/g 6-bromoisatin. All HBG draw out and 6-bromoisatin treated mice display maintained alveolar space and parenchymal architecture with thin alveolar walls (green arrow mind) and lack of indications of haemorrhage or Axitinib tyrosianse inhibitor macrophage and neutrophil infiltration. Axitinib tyrosianse inhibitor Images are representative of 3 different sections from six mice per group magnified 400X and level bars arranged to 20 m.(TIF) pone.0186904.s002.tif (1.2M) GUID:?74B826FD-7E59-4E26-BD42-79F128099034 S3 Fig: Correlations between histological parameters in the lungs of mice. Axitinib tyrosianse inhibitor The relationship between the overall histopathological score and A) neutrophil infiltration; B), macrophage infiltration; C) vascular congestion; D) alveolar damage. The linear relationship and R2 ideals are identified from all samples pooled across all mice in the LPS-stimulated acute lung swelling model: PBSCve bad control; LPS +ve lipopolysaccharide stimulated positive control; HBGE Hypobranchial gland draw out at 0.1 and 0.5 mg/g and; 6Br 6 bromoisatin at 0.1 and 0.05 mg/g. ** significant relationship at p 0.01.(TIF) pone.0186904.s003.tif (350K) GUID:?8D75ACB4-5485-48C4-BEFA-0C9C2741A61C S4 Fig: Correlations between neutrophil infiltration and inflammatory markers in the lungs of mice. The relationship between the histopathological score for neutrophils and Rabbit polyclonal to FBXO42 guidelines in the bronchoalveolar lavage fluids: A) Neutrophil count; B), total cell count (TCC); C) Tumor necrosis element (TNF) alpha; D) interleukin I-1B; and E) protein. The linear relationship and R2 ideals are identified from all samples pooled across all mice in the LPS-stimulated acute lung swelling model: PBSCve bad control; LPS +ve lipopolysaccharide stimulated positive control; HBGE Hypobranchial gland draw out at 0.1 and 0.5 mg/g and; 6Br 6 bromoisatin at 0.1 and 0.05 mg/g. ** significant relationship at p 0.01.(TIF) pone.0186904.s004.tif (320K) GUID:?DD5435F9-CE29-46A1-905E-AD3F1103B50D S5 Fig: Correlations between macrophages and inflammatory markers in the lungs of mice. The relationship between the histopathological score for macrophages infiltration and guidelines in the bronchoalveolar lavage fluids: A) Neutrophil count; B), total cell count (TCC); C) Tumor necrosis element (TNF) alpha; D) interleukin I-1B; and E) protein. The linear relationship and R2 ideals are identified from all samples pooled across all mice in the LPS-stimulated acute lung swelling model: PBSCve bad control; LPS +ve lipopolysaccharide stimulated positive control; HBGE Hypobranchial gland draw out at 0.1 and 0.5 mg/g and; 6Br 6 bromoisatin at 0.1 and 0.05 mg/g. ** significant relationship at p 0.01.(TIF) pone.0186904.s005.tif (297K) GUID:?0927F86C-46F7-47C2-A836-76BD8712FBA7 S6 Fig: Correlations between alveolar destruction and inflammatory markers in the lungs of mice. The relationship between the histopathological score for alveolar damage and guidelines in the bronchoalveolar lavage fluids: A) Neutrophil count; B), total cell count (TCC); C) Tumor necrosis element (TNF) alpha; D) interleukin I-1B; and E) protein. The linear relationship and R2 ideals are identified from all samples pooled across all mice in the LPS-stimulated acute lung swelling model: PBSCve bad control; LPS +ve lipopolysaccharide stimulated positive control; HBGE Hypobranchial gland draw out at 0.1 and 0.5 mg/g and; 6Br 6 bromoisatin at 0.1 and 0.05 mg/g. ** significant relationship at Axitinib tyrosianse inhibitor p 0.01.(TIF) pone.0186904.s006.tif (332K) GUID:?3E9FE408-01AE-4109-88DE-02EC718B8214 S7 Fig: Correlations between vascular congestion and inflammatory markers in the lungs of mice. The relationship between the histopathological score for vascular congestion and guidelines in the bronchoalveolar lavage fluids: A) Neutrophil count; B), total cell count (TCC); C) Tumor necrosis element (TNF) alpha; D) interleukin I-1B; and E) protein. The linear relationship and R2 ideals are identified from all samples pooled across all mice in the LPS-stimulated acute lung swelling model: PBSCve bad control; LPS +ve lipopolysaccharide stimulated positive control; HBGE Hypobranchial gland draw out at 0.1 and 0.5 mg/g and; 6Br 6 bromoisatin at 0.1 and 0.05 mg/g. ** significant relationship.