Silver precious metal nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years. AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties. Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability. In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells. The film shows great potential as a novel kind of wound dressing. ((and (a) and (b). Red dotted circle represents the edge of the inhibition zone. Table 1 Diameters of the inhibition zones of SS/Agar, PDA-SS/Agar and AgNPs-PDA-SS/Agar films against (a) and (b). (Figure 8a) and (Figure 8b) in the presence of different films, respectively. The growth of and in the presence of SS/Agar and PDA-SS/Agar films was similar to the control, indicating that SS/Agar and PDA-SS/Agar films did not have bacteriostatic activity. Compared with the Dexamethasone tyrosianse inhibitor control, AgNPs-PDA-SS/Agar significantly inhibited bacterial growth up to 20 h, recommending that AgNPs-PDA-SS/Agar film got a efficient and long-term inhibition influence on bacterial growth. Open up in another window Shape 8 Bacterial development curve of (a) and (b) in the current presence of different movies, and antimicrobial balance evaluation of AgNPs-PDA-SS/Agar film under different pH circumstances (c,d). 2.8. Antimicrobial Balance AgNPs-PDA-SS/Agar film was treated at different pH (4.0, 7.4, 10.0) for 24 h, and the inhibitory aftereffect of the treated film against and was determined. As demonstrated in Shape 8c,d, in the lack of AgNPs, there is no factor in bacterial development between SS/Agar as well as the control at different period factors, indicating SS/Agar film got no bacteriostasis capability. Weighed against the control, the bacterial development was certainly inhibited in the current presence of AgNPs-PDA-SS/Agar film after treatment with different pH, recommending AgNPs-PDA-SS/Agar film got long-term and steady antibacterial capability, which was beneficial for wound Dexamethasone tyrosianse inhibitor dressing and additional potential applications. 2.9. Cytocompatibility To judge the cytotoxicity of SS/Agar, AgNPs-PDA-SS/Agar and PDA-SS/Agar films, cell keeping track of package-8 (CCK-8) assay was performed to examine the cells treated with different movies. In the check, the metabolically energetic cells react using the tetrazolium sodium in the CCK-8 remedy to make a soluble formaldehyde nitrogen dye with optimum absorbance at 450 nm [39]. Optical denseness (OD) demonstrates cell success and living cells [40]. The outcomes showed there is no factor in cell viability between your control as well as the experimental group treated with AgNPs-PDA-SS/Agar film (Shape 9). Notably, the cell viability when treated with PDA-SS/Agar film was greater than that of the control, indicating PDA had not been only nontoxic on cells, but could promote cell proliferation to boost cell viability also. Furthermore, the cell morphology under different remedies almost didn’t modification after 24 h (Shape 10), suggesting how the ready movies had superb cytocompatibility for the fibroblast NIH/3T3 cells, which is beneficial for its application in biomaterials. Open in a separate window Figure 9 CCK-8 assay of the cytocompatibility of different films on NIH/3T3 cells. The statically significant values are expressed by NS (not significant), ( 0.05), ( 0.01) and ( 0.001). Open in a separate window Figure 10 Microscopic observation of NIH/3T3 cells morphology with control (a), in the presence of SS/Agar film (b), PDA-SS/Agar film (c) and AgNPs-PDA-SS/Agar film (d). Small box represents a selected area, big box represents the enlarged image in the small box. PIK3CG White arrows indicate the observed fibroblast NIH/3T3 cells. The scale bar is 400 m. To better visualize the effects of the prepared films on NIH/3T3 cells viability, a living/dead cell staining assay was performed. In this assay, living cells are stained green, while dead cells are red. After being treated with different films for 24 h, the fluorescence images clearly showed almost all cells were stained green, a very few cells ( 1) were stained red (marked with white arrows, Figure 11), indicating the excellent cytocompatibility of the films on NIH/3T3 cells. This result was in good agreement with Dexamethasone tyrosianse inhibitor that of CCK-8 assay and the microscopic observation Dexamethasone tyrosianse inhibitor Dexamethasone tyrosianse inhibitor on cell morphology. Open in a separate window Figure 11 Living/dead cell staining assay of NIH/3T3 cells after being treated with different films. White arrows indicate a very few cells ( 1) were stained red..