We display that, at low forces, the force needed to deform growing cells to the same extent as non-growing cells is definitely approximately two times smaller. (a) midline position for a non-growing cell before (gray) and after (black) the application of a push (infusion rate is definitely ). Straight gray line TCN238 represents the end of the growth channel. (b) Results of the custom code written in Matlab for the analysis of the deformation. Black collection – conformation of the part of the cell from (a) in the main channel in reduced coordinates for which the total arclength of the cell is definitely . Dark gray collection – conformation of a cell as deduced from your elastic equations for which the angle at the base and the angle at the tip are equals to these of the analyzed cell. Light gray lines – same as the dark gray line with the angle at the base and the angle at the tip equals to the fitted ideals plus and minus the error of the suits respectively.(TIF) pone.0083775.s004.tif (1.1M) GUID:?2A6785FD-B46D-446D-98A0-F5278DFBF821 Number S5: Profile of the deformation of growing cells. (a)-(f) for infusion rates of , , , , and and for , ,, , and cells, respectively. was recorded at different time points during the growth of cells when a push was constantly applied on them. Gray lines are suits to a linear function of the monotonically increasing portion of .(TIF) pone.0083775.s005.tif (1.5M) GUID:?29088E74-5567-4BEC-8881-44BE7C25173A Number S6: Velocity profile in the main channel. (a) Example of the velocity profile in the main channels. The infusion rate was . Different colours represent measurements of the velocity profile in the remaining and right sides of the two main channels. For each position, next to the growth channels a short boundary zone was observed where the velocity decreased. Further away from the growth channels a plateau of the value of the velocity was observed. (b) Velocity over the maximum velocity at that height. Black curve theoretical value. Gray curve average of the four curves from (a).(TIF) pone.0083775.s006.tif (2.6M) GUID:?91574B84-F6E7-4AAE-9143-53C127E92697 Figure S7: Flow velocity like a function of the infusion rate. Measured plateau velocity of beads like a function of the infusion rate. Blue circles and reddish triangles represents the results of two different experiments. For assessment theoretical values, TCN238 based on the measured dimensions of the main channels, and presuming a homogeneous circulation profile are demonstrated (green dots). The theoretical value is definitely larger than the measured value, a truth that is consistent with the non-homogenous circulation profile inside the main channel.(TIF) pone.0083775.s007.tif (7.2M) GUID:?55156A47-E028-489D-AABC-659B0D50AC37 Figure S8: Theoretical circulation profile in the device based on Gondret et al. [39] . (A) Expected normalized circulation profile inside a close duct having a cross section of . Velocities were normalized to the maximal velocity at the center of the channel. (B) Predicted Rabbit polyclonal to Neurogenin1 normalized circulation profile inside a close duct, with the above mentioned dimensions, in the relative part of the channel the cell in our experiment may occupy. Velocities were normalized to the maximal velocity at the center of the channel. (C) Expected circulation velocities on the velocity at the same point at a height of in the relative part of the channel the cell in our experiment may occupy. Baseline was chosen to become at half of the cells diameter, thus giving an estimation of the velocity that a longitudinal section of the cells encounter relative to the velocity we measured.(png) pone.0083775.s008.png (737K) GUID:?0A24133A-C54C-4866-A911-E290378BB914 Movies S1: Examples of the circulation around cells. micron beads (reddish) were infused into the microfluidic device in the presence of cells (green). Notice how the trajectories of the beads are affected by the presence of the cells only adjacent to the cells themselves. Exposure time of each frame in the red channel . Delay between frames min, infusion rate .(AVI) pone.0083775.s009.avi (2.5M) GUID:?BCF5ACAC-3D12-4373-ACFD-204473A5F500 TCN238 Movies S2: Examples of the flow around cells. micron beads (reddish) were infused into the microfluidic device in the presence of cells (green). Notice how the trajectories of the beads are affected by the presence of the cells only adjacent to the cells themselves. Exposure time of each frame in the red channel . Delay between frames min, infusion rate .(AVI) pone.0083775.s010.avi (417K) GUID:?43E2F1CB-8E82-448F-B974-A31646A6A618 Movies S3: Trajectories of beads in empty device. Trajectories of 0.5 micron beads in the microfluidic device as recorded by a.