Supplementary MaterialsFIGURE S1: Effect of normal glucose (G) and high glucose (HG) about Bcl-2, Bcl-XL, Bax, PARP, and cyt c protein expression in absence or presence of 5-FU in human being colon cancer HT29 cells. 25 and 50 M, respectively, 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; ?? 0.001 50 M 5-FU in HT29 cells cultured in HG vs. 50 M 5-FU in HT29 cells cultured in G. (B) For Bcl-XL measurements: ?? 0.001 25 and 50 M, respectively, 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; 0.001 0 M 5-FU in HT29 cells cultured in HG vs. 0 M 5-FU in HT29 cells cultured in G; ?? 0.01 25 and 50 M 5-FU in HT29 cells cultured in HG vs. 25 and 50 M 5-FU in HT29 cells cultured in G. (C) For Bax measurements: ??? 0.0001 50 M 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; 0.001 0 M 5-FU in HT29 cells cultured in HG vs. 0 M 5-FU in HT29 cells cultured in G; 0.002 and 0.001 25 and 50 M, respectively, 5-FU in HT29 cells cultured in HG vs. 0 M 5-FU X in HT29 cells cultured in HG; TL32711 cost ?? 0.001 50 M 5-FU in HT29 cells cultured in HG vs. 50 M 5-FU in HT29 cells cultured in G. (D) For Bax/Bcl-2 percentage measurements: ? 0.001 and ??? 0.0001 25 and Rabbit Polyclonal to RUFY1 50 M, respectively, 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; ?? 0.001 50 M 5-FU in HT29 cells cultured in HG vs. 50 M 5-FU in HT29 cells cultured in G. (E) For Bax/Bcl-XL percentage measurements: ? 0.01 and ??? 0.001, respectively, 25 and or 50 M 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; 0.001 0 M 5-FU in HT29 cells cultured in HG vs. 0 M 5-FU in HT29 cells cultured in G; ?? 0.001 and ??? 0.0001, respectively, 25 and or 50 M 5-FU in HT29 cells cultured in HG vs. 25 and or 50 M 5-FU in HT29 cells cultured in G. (F) For full size PARP measurements: ?? 0.001 and ??? 0.0001 25 and 50 M, respectively, 5-FU in HT29 cells cultured in G vs. 0 M 5-FU in HT29 cells cultured in G; ?? 0.001 respectively, 25 and or 50 M 5-FU in HT29 cells cultured in HG vs. 25 and or 50 M 5-FU in HT29 cells cultured in G. (H) For cit cyt c measurements: ??? 0.0001 TL32711 cost 25 and 50 M, respectively, 5-FU in HT29 cells cultured TL32711 cost in G vs. 0 M 5-FU in HT29 cells cultured in G; 0.001 0 M 5-FU in HT29 cells cultured in HG vs. 0 M 5-FU in HT29 cells cultured in G; 0.001 25 and 50 M 5-FU in HT29 cells cultured in HG vs. 0 M DOX in HT29 cells cultured TL32711 cost in HG; ?? 0.0001 25 and 50 M 5-FU, respectively, in HT29 cells cultured in HG vs. 25 and 50 M 5-FU, respectively, DOX in HT29 cells cultured in G. (G,I) For GAPDH and -tubulin measurements: n.s. Image_2.JPEG (5.4M) GUID:?F31D2AE0-53C7-4684-B17F-38B8DC7B0BE2 FIGURE S3: Effect of normal glucose (G) and high glucose (HG) about Bcl-2, Bcl-XL, Bax, PARP, and cyt.